Regulatory

Part:BBa_K4451018:Design

Designed by: Brooks J Rady   Group: iGEM22_Sheffield   (2022-09-30)


pJKR PLtetO-1 + RBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 74
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 74
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 74
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 74
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 74
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References

Rogers, J. K. et al. Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 43, 7648–7660 (2015).